Journal: Molecular Oncology

Article Title: SNAIL1 combines competitive displacement of ASCL2 and epigenetic mechanisms to rapidly silence the EPHB3 tumor suppressor in colorectal cancer

doi: 10.1016/j.molonc.2014.08.016

Figure Lengend Snippet: Snail1‐HA completely disables the EPHB3 enhancer. (A–C) ChIP analyses of Snail1‐HA (A), TCF7L2 (B) and H3K4me1 (C) occupancy at the EPHB3 locus in derivatives of LS174T cells 96 h after Snail1‐HA induction. n ≥ 3. ...

Article Snippet: Cell culture The CRC cell lines LS174T (CLS #300392) and HT29 (CLS #300215) were obtained from the Cell Line Service culture collection (DKFZ, Heidelberg) and ATCC, respectively.

Techniques:

Journal: Molecular Oncology

Article Title: SNAIL1 combines competitive displacement of ASCL2 and epigenetic mechanisms to rapidly silence the EPHB3 tumor suppressor in colorectal cancer

doi: 10.1016/j.molonc.2014.08.016

Figure Lengend Snippet: Snail1‐HA engages HDACs and LSD1 to repress EPHB3. (A,B) ChIP analyses of H3ac, H3K4me3 (A) and HDAC1 occupancy (B) in derivatives of LS174T cells. n ≥ 3. (C) qRT‐PCR analyzing HDAC1, HDAC2 and EPHB3 expression ...

Article Snippet: Cell culture The CRC cell lines LS174T (CLS #300392) and HT29 (CLS #300215) were obtained from the Cell Line Service culture collection (DKFZ, Heidelberg) and ATCC, respectively.

Techniques: Quantitative RT-PCR, Expressing

Journal: Molecular Oncology

Article Title: SNAIL1 combines competitive displacement of ASCL2 and epigenetic mechanisms to rapidly silence the EPHB3 tumor suppressor in colorectal cancer

doi: 10.1016/j.molonc.2014.08.016

Figure Lengend Snippet: Snail1‐HA expression interferes with marker gene expression of intestinal epithelial stem cells and self‐renewal capacity. (A) qRT‐PCR analyses of the genes shown in derivatives of LS174T cells. Expression (expr.) levels are shown ...

Article Snippet: Cell culture The CRC cell lines LS174T (CLS #300392) and HT29 (CLS #300215) were obtained from the Cell Line Service culture collection (DKFZ, Heidelberg) and ATCC, respectively.

Techniques: Expressing, Marker, Gene Expression, Quantitative RT-PCR

Journal: Molecular Oncology

Article Title: SNAIL1 combines competitive displacement of ASCL2 and epigenetic mechanisms to rapidly silence the EPHB3 tumor suppressor in colorectal cancer

doi: 10.1016/j.molonc.2014.08.016

Figure Lengend Snippet: Sustained expression of EPHB3 interferes with Snail1‐HA‐induced EMT. (A) Scheme illustrating the construction of LS174T CRC cell derivatives. In a first step, LS174T cells were tranduced with a retroviral vector for Dox‐inducible ...

Article Snippet: Cell culture The CRC cell lines LS174T (CLS #300392) and HT29 (CLS #300215) were obtained from the Cell Line Service culture collection (DKFZ, Heidelberg) and ATCC, respectively.

Techniques: Expressing, Retroviral, Plasmid Preparation

Journal: Molecular Oncology

Article Title: SNAIL1 combines competitive displacement of ASCL2 and epigenetic mechanisms to rapidly silence the EPHB3 tumor suppressor in colorectal cancer

doi: 10.1016/j.molonc.2014.08.016

Figure Lengend Snippet: EPHB3 counteracts Snail1‐HA‐induced EMT in vivo. Representative images of serial sections of xenograft tumors formed by LS174T control cells or derivatives expressing Snail1‐HA and Snail1‐HA in the continuous presence ...

Article Snippet: Cell culture The CRC cell lines LS174T (CLS #300392) and HT29 (CLS #300215) were obtained from the Cell Line Service culture collection (DKFZ, Heidelberg) and ATCC, respectively.

Techniques: In Vivo, Control, Expressing

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: In vitro characterization of new anti-CEA antibodies. (a) SPR sensorgram of G9, F7, and F4 in a scFv format. K D values were calculated to be 640 nM, 50 nM, and 7.7 nM, respectively. (b) Flow cytometry analysis with the new antibodies in IgG format on CEA-expressing CT26 cells and on CEA-negative CT26 wild-type cells. (c) Immunofluorescence staining with IgG formats on the human colon adenocarcinoma xenograft LS174T. Anti-CEA antibodies were detected in green. Blood vessels were detected by CD31 staining (red). 20× magnification, scale bars = 100 μm.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: In Vitro, Flow Cytometry, Expressing, Immunofluorescence, Staining

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: Ex vivo immunofluorescence-based biodistribution analysis. Immunofluorescence analysis assessed tumor targeting of new anti-CEA antibodies in IgG format. Two hundred micrograms of IgG-FITC were injected intravenously into LS174T-bearing mice. Tumors were excised 24 hours after injection. IgG-FITC was detected in green; blood vessels were detected through CD31 staining (red). 20× magnification, scale bars = 100 μm.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: Ex Vivo, Immunofluorescence, Injection, Staining

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: Quantitative biodistribution with radiolabeled anti-CEA antibodies in diabody format. Quantitative biodistribution analysis of radio iodinated anti-CEA diabodies in BALB/c nude mice bearing subcutaneous LS174T colon adenocarcinomas. Organs were harvested 24 hours after intravenous injection, and radioactivity was quantified. Results are shown as the percentage of injected dose per gram (ID/g (%)). Error bars = SEM; n = 4.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: Injection, Radioactivity

Journal: Hormones & Cancer

Article Title: Targeting the Mitotic Checkpoint to Kill Tumor Cells

doi: 10.1007/s12672-010-0059-x

Figure Lengend Snippet: Conditional inactivation of Mps1 causes inactivation of the mitotic checkpoint and tumor cell death. a Left LS174T-TetRMps1, treated without (−) and with (+) doxycycline (dox) for 3 days, were immunoblotted for Mps1 and α-tubulin. Right colony formations of LS174T-TetRMps1 cells treated with and without dox for 11 days. b FACS analysis of LS174T-TetRMps1 cells treated with or without dox for 3 days and 1 μM taxol for 18 h. The percentage of mitotic cells was measured as the fraction of cells with 4N DNA content that were positive for MPM2

Article Snippet: LS174T cells were grown in DMEM (Lonza) with 10% Tet-approved FCS (Clontech), supplemented with pen/strep (Invitrogen) and ultraglutamine (Lonza).

Techniques:

Journal: Hormones & Cancer

Article Title: Targeting the Mitotic Checkpoint to Kill Tumor Cells

doi: 10.1007/s12672-010-0059-x

Figure Lengend Snippet: Conditional inactivation of Mps1 causes severe defects in chromosome segregation and severe aneuploidy. a LS174T-TetRMps1 cells were treated with or without dox for 3 days and fixed after 90 min of MG132 treatment. No alignment defects , mild alignment defects , or severe alignment defects indicate mitotic cells with zero, one to five, or more than five chromosomes not aligned on the metaphase plate, respectively. b Chromosome spreads were performed on cells after 4 days of treatment with or without dox. At least 60 chromosome spreads were counted per condition

Article Snippet: LS174T cells were grown in DMEM (Lonza) with 10% Tet-approved FCS (Clontech), supplemented with pen/strep (Invitrogen) and ultraglutamine (Lonza).

Techniques:

Journal: ACS Omega

Article Title: Microbubble Enhanced Delivery of Vitamin C for Treatment of Colorectal Cancer

doi: 10.1021/acsomega.4c06779

Figure Lengend Snippet: LS174TIn VitroCell Response to PAL. LS174T cell viability following 1 h exposure to PAL and BL. The bar chart shows treatment with PAL compared to the carrier (BL), which used the same liposome preparation method and yielded a similar liposome concentration but omitted PA ( n = 3, error bars represent standard error for three biological repeats).

Article Snippet: LS174T human Caucasian colon adenocarcinoma (ECACC 87060401) and HCT116 human colon carcinoma (ECACC 91091005) cells were initially obtained from the European Collection of Authenticated Cell Cultures (ECACC, UK).

Techniques: Concentration Assay

Journal: ACS Omega

Article Title: Microbubble Enhanced Delivery of Vitamin C for Treatment of Colorectal Cancer

doi: 10.1021/acsomega.4c06779

Figure Lengend Snippet: Fluorescence maps and analysis of LS174T cells cultured on-chip and treated with PAL, MBs, and US. Treatment with (a) culture media only, or (b) PAL + MB + US (5×). Histograms showing the distribution of live and dead stained cells (dead pixel count multiplied by 10 to aid visualization), corresponding to the confocal images of LS174T cells post-treatment with (c) culture media or (d) PAL + MB + US (5×). Histograms showing the distribution of the fraction of dead LS174T cells (dead pixel count/total pixel count) which received treatment with (e) culture media only or (f) PAL + MB + US (5×).

Article Snippet: LS174T human Caucasian colon adenocarcinoma (ECACC 87060401) and HCT116 human colon carcinoma (ECACC 91091005) cells were initially obtained from the European Collection of Authenticated Cell Cultures (ECACC, UK).

Techniques: Fluorescence, Cell Culture, Staining

Journal: ACS Omega

Article Title: Microbubble Enhanced Delivery of Vitamin C for Treatment of Colorectal Cancer

doi: 10.1021/acsomega.4c06779

Figure Lengend Snippet: LS174T cell viability post BL, PAL, MB, and US exposures on-chip. (a) Cell viability data for LS174T cells on-chip, post-treatment with BL, PAL (5 mM), MB (1 × 10 8 MBs/mL), and US combinations ( n > 3 for all conditions, except for PAL (No US), conducted twice; error bars represent the standard error). Confocal images of stained LS174T cells following treatment with PAL + MB + US (5×) (b) inside and (c) outside of the US-exposed region. Confocal images of stained LS174T cells treated with culture media (DMEM) in the channel’s (d) central and (e) edge regions.

Article Snippet: LS174T human Caucasian colon adenocarcinoma (ECACC 87060401) and HCT116 human colon carcinoma (ECACC 91091005) cells were initially obtained from the European Collection of Authenticated Cell Cultures (ECACC, UK).

Techniques: Staining